Composite

Part:BBa_K4800043:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-08)


pTrc99a-SAV2585


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4486
    Illegal PstI site found at 3174
    Illegal PstI site found at 4498
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4486
    Illegal PstI site found at 3174
    Illegal PstI site found at 4498
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 3374
    Illegal NgoMIV site found at 3403
    Illegal NgoMIV site found at 3448
    Illegal NgoMIV site found at 4252
    Illegal NgoMIV site found at 4366
    Illegal AgeI site found at 3059
    Illegal AgeI site found at 3110
    Illegal AgeI site found at 3185
    Illegal AgeI site found at 3299
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4682
    Illegal BsaI site found at 5750
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References